Correlation between Specific Carcinogens and Mitotic Defects in Uterine Cancer.

Author

• Leonid Zlotcavitch

Date of Award

2011

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Quintyne, Nicholas

Abstract

The development of uterine cancer and its progression can be linked to external elements such as carcinogens, natural hormones and chemotherapeutic drugs. The goal of this project was to investigate the possible correlation between exposure to specific chemical and an increase of mitotic defects. These defects manifest themselves as lagging chromosomes, multipolar spindles, and anaphase bridges. Using the uterine cancer cell line MES-SA as a model, we established a baseline for the mitotic defects in the absence and presence of various chemicals using γ-tubulin and DAPI-immune stained fixed cells. The cells were treated with varying concentrations of Vinyl Chloride, β-estradiol, Fluvestrant, and were examined using Immunofluorescent microscopy. We found that an exposure to Vinyl Chloride, β-estradiol and Fulvestrant have increased the number of mitotic defects in this cell line.

Comments

Includes bibliography.

Recommended Citation

Zlotcavitch, Leonid, "Correlation between Specific Carcinogens and Mitotic Defects in Uterine Cancer." (2011). Harriet L Wilkes Honors College Theses 2010 - 2019. 90.
https://digitalcommons.fau.edu/etd_honors_2/90

Extent

34 p.

Identifier

FA00003611_0