Isolation of the methionine sulfoxide reductase B3 (MSRB3) gene from the red-eared slider, Trachemys scripta elegans

Author

• Eric Bishop

Date of Award

2011

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Kirchman, Paul A.

Abstract

Oxidative stress from reactive oxygen species (ROS) is hypothesized to be a primary cause of aging and aging-related diseases. For my thesis research, my advisor and I examined adaptation to oxidative stress in the red-eared slider, Trachemys scripta elegans, a turtle that goes long periods with low oxygen during hibernation, but then quickly re-acclimates to higher oxygen levels upon surfacing. This dramatic ischemia/reperfusion event should result in ROS formation, but the turtle appears to reduce oxidative stress. We aimed to isolate the gene for methionine sulfoxide reductase B3, a protein that alleviates oxidative stress by reconverting oxygen damaged methionine-R-sulfoxide to the amino acid methionine. We designed degenerate primers to amplify a conserved region of the MSRB gene to isolate a partial DNA sequence. We then used 5’ RACE PCR and vectorette library PCR to isolate much of the coding region of the gene from T. scripta.

Comments

Includes bibliography.

Recommended Citation

Bishop, Eric, "Isolation of the methionine sulfoxide reductase B3 (MSRB3) gene from the red-eared slider, Trachemys scripta elegans" (2011). Harriet L Wilkes Honors College Theses 2010 - 2019. 39.
https://digitalcommons.fau.edu/etd_honors_2/39

Extent

51 p.

Identifier

FA00003560