Date of Award

2018

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Grill, Brock

Abstract

EEL-1, the HUWE1 ortholog in C. elegans, is a large E3 ubiquitin ligase that is involved in the regulation of neuronal function and development. Huwe1 has been shown to be associated with intellectual disability. Through the use of electrophysiology and cell specific rescue it has been shown that EEL-1 is involved in GABAergic synaptic transmission. The mechanism of action of EEL-1 within GABA neurons remains largely unknown. Affinity purification proteomics have identified OGT-1, an ortholog of mammalian O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt), as an EEL-1 binding protein. By analyzing the expression pattern of OGT-1 in transgenic animals, we show that OGT-1 is expressed both GABAergic motor neurons and cholinergic motor neurons. Analysis of the subcellular distribution within GABAergic motor neurons shows that OGT-1 is enriched in presynaptic terminals. These findings support the hypothesis that EEL-1 and OGT-1 form a complex within GABA motor neurons to regulate synaptic transmission.

Comments

Includes bibliography.

Extent

22 p.

Identifier

FAUHT00048

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