Date of Award

2017

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Moore, Jon

Abstract

Seafood is a main protein source for millions of people, making up a great extent of the world’s diet. Seafood sales are also the foundation to the economies of many communities and villages worldwide. Unfortunately, a great portion of these sales are mislabeled causing potential health, economic, and species population problems. Eight highly rated restaurants in the Jupiter, Florida area were investigated for potential fish fraud. Sashimi samples were collected from each restaurant and then taken to the lab for testing. DNA was extracted using a BIO RAD DNA Barcoding kit, which targets the mitochondrial COI gene. The PCR products produced by the barcoding kit were then sent to Sequetech Lab for sequencing. After sequencing was finalized, species identification was possible using the Barcode of Life Database (BOLD). Results are being used to determine the validity of food labeling by of local seafood restaurants and inform consumers of the potential problems that could result from purchasing mislabeled food.

Comments

Includes bibliography.

Extent

20 p.

Identifier

FA00012619

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