Date of Award

2017

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Young, Samuel M.

Abstract

Helper-Dependent Adenoviruses (HdAd) are the ideal viral vector choice for expression of Munc13 proteins due to the large packaging capacity needed (~9.8 kb) for the triple expression cassettes of Munc13, iCre, and EGFP. Development of a HdAd vector for the expression of Munc13 gene family proteins allows for the rapid expression of molecules located at the synaptic active zone critical for neurotransmitter release. The Munc13 HdAd viral vector can be used to express proteins of the munc13 gene family in the calyx of Held. This will allow for the elucidation of the molecular mechanisms employed by the calyx of Held in the process of encoding the onset and modulation of sound. Production of an HdAd vector for the expression of Munc13 proteins occurs in five parts; cloning, transfection, amplification, purification and titering. After production of the HdAd vector, Munc13 protein expression was analyzed by western blot.

Comments

Includes bibliography.

Extent

24 p.

Identifier

FA00012631

Download

Share

COinS