Date of Award

2016

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Steinecke, Andre

Abstract

Recent findings show it is possible to genetically target Chandelier Cells (ChC) by using transgenic animals expressing a CRE recombinase under the control of a transient transcription factor. This targeting allows for continuous expression of a Green Fluorescent Protein (GFP) to study ChCs intrinsic cellular processes. However, the expression of GFP in these mice is weak and the transient transcription factor limits the ability to achieve cell type specific expression of genes. To overcome these problems a technique of transplanting transfected ChC-progenitors into the cortex of developing mice, using single cell electroporation, was created. To compare both methods an analysis of the axon arborization and innervation of ChCs on post-natal day 16 and 21 in mice brains was done. The results show there is not a significant difference between the transplanted and intrinsic signal brains, and that the process of transplantation is a viable method for studying the ChC development.

Comments

Includes bibliography.

Extent

28 p.

Identifier

FA00012604

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