Some like it hot: the isolation of an aconitase mutant resistant to heat shock-induced oxidative stress

Author

• Imarhia Enogieru

Date of Award

2013

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Kirchman, Paul

Abstract

Aconitase is a multifunctional enzyme essential for mitochondrial DNA maintenance and cellular respiration. For my thesis, I used an error prone PCR mutagenesis method to mutate the yeast aconitase (ACO1) gene and created a mutant library of Sacchromyces cerevisiae yeast cells. The library underwent 50oC heat shocks to select for cells expressing an aconitase mutant that increased the cells’ resistance to heat shock-induced oxidative stress. Oxidative stress has been implicated as a theory of cellular aging. Isolated aconitase mutants with increased oxidative stress resistance will be assayed for increased lifespan, which would support the free radical theory of aging and implicate aconitase as an important determinant of cellular aging.

Comments

Includes bibliography.

Recommended Citation

Enogieru, Imarhia, "Some like it hot: the isolation of an aconitase mutant resistant to heat shock-induced oxidative stress" (2013). Harriet L Wilkes Honors College Theses 2010 - 2019. 145.
https://digitalcommons.fau.edu/etd_honors_2/145

Extent

26 p.

Identifier

FA00003511