Author Type

Undergraduate Student

Date of Award

Spring 5-2-2022

Document Type

Thesis

Publication Status

Version of Record

Submission Date

June 2026

College Granting Degree

Harriet L. Wilkes Honors College

Thesis/Dissertation Advisor [Chair]

Randy Blakely

Abstract

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating, multisystem disease characterized by severe fatigue without medical explanation. Due to the complexity of the disease, treatments only partially alleviate symptoms and currently there is no cure. To develop more effective treatments and better understand its etiology, valid preclinical models need to be developed. Evidence suggests infection and serotonin homeostasis plays a role in the development of ME/CFS. Therefore, I aimed to examine whether a viral mimetic (Poly I:C) triggers long-term fatigue behavior and neuroinflammation in a mouse model. C57 and CD1 male mice were treated with Poly I:C and only CD1 mice demonstrated prolonged fatigue behavior. However, C57 mice showed increase expression of the serotonin transporter (SERT) in the brain and periphery, whereas CD1 mice showed no changes in SERT or markers of inflammation. Continued optimization of this model is required to determine under what conditions infection can produce prolonged fatigue.

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