Date of Award

2022

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Duboué, Erik

Abstract

Fluorescent in-situ hybridization chain reaction (HCR-RNA FISH) is a technique that allows for the simultaneous labeling of multiple target mRNA molecules, signal quantification, and high-resolution oligonucleotide visualization. While this method has been employed to study several common laboratory model systems, its application to non-traditional models is lacking. With the goals of circumventing this limitation and introducing Hybridization Chain Reaction (v3.0) (HCR), the latest version of a new technique for high resolution in situ hybridization, to the Astyanax mexicanus model, we have optimized a novel protocol for this robust evolutionary system. The optimization of HCR in Astyanax mexicanus strengthens the reproducibility of this staining method across a larger number of model organisms while opening the doors to the molecular physiology and genetic study of an insufficiently explored yet promising biological system.

Comments

Thesis (B.S.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2022

Extent

48 p.

Identifier

FAUHT00229

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