Date of Award
2012
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Abstract
We employed three genotypes of GAD 65, wildtype (GAD 65 +/+), heterozygous (GAD 65 +/-) and knockout (GAD 65 -/-) to investigate the role of GAD 65 in survival of pancreatic islets. We analyzed the mRNA expression of pro-survival proteins including Bcl2 and Bax in pancreas of wildtype, heterozygous and knockout using Reverse Transcriptase Polymerase Chain Reaction (RTPCR). The level of expression of Bcl2 mRNA was down regulated in knockout mice pancreas and Bax to Bcl2 ratio was found higher in knockout mice pancreas suggesting higher cell death rate. However, further studies are required to recognize and understand the specific connections between apoptotic pathways and GAD 65 in pancreatic islets.
Extent
ix, 37, [vi] p. : ill. (some col.)
Recommended Citation
Kumari, Neeta., "GAD 65 and its role in pancreatic tissue survival" (2012). Electronic Theses and Dissertations 2010 - 2019. 524.
https://digitalcommons.fau.edu/etd_general_6/524
Identifier
FADT3342206
Comments
by Neeta Kumari.|Thesis (M.S.)--Florida Atlantic University, 2012.|Includes bibliography.|Electronic reproduction. Boca Raton, Fla., 2012. Mode of access: World Wide Web.|by Neeta Kumari.