Author Type

Graduate Student

Date of Award

2018

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Louda, J. W.

Abstract

This project was designed to investigate the effects of carbon dioxide (CO2) levels on the growth and pigment ratios (chemotaxonomy) of freshwater algal species typical to the south Florida surface waters. Green algae, diatoms, and cyanobacteria were cultured under 400 or 800 ppm CO3 in air for several weeks. Growth monitoring used a cell counter, hemocytometer, and chlorophyll fluorescence. Pigments were analyzed using HPLC-PDA. Experiments with certified CO2 concentrations (400, 600, 800, 1200 ppm) were conducted with helium degassed ultrapure water and each of three culture media. Theoretical and experimental pH values with water matched exactly. However, each culture media proved to exhibit significant buffer capacity. Cell growth monitoring was problematic except for the cyanobacterium Microcystis aeruginosa. That species responded to increased CO2 (800 ppm) with increased growth rates as predicted. The other species gave erratic results mainly due to difficulties in obtaining valid consistent cell counts.

Extent

83 p.

Comments

Includes bibliography.|Thesis (M.S.)--Florida Atlantic University, 2018.|FAU Electronic Theses and Dissertations Collection|Includes bibliography.

Identifier

FA00013170

Format

pdf

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