Author Type

Graduate Student

Date of Award

2018

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Zhuang, Hanqi

Thesis/Dissertation Co-Chair

Du, Sarah

Abstract

Sickle cell disease is an inherited blood cell disorder that affects about 100,000 people in the US and results in high cost of medical care exceeding $1.1 billion annually. Sickle cell patients suffer from unpredictable, painful vaso-occlusive crises. Portable, costeffective approaches for diagnosis and monitoring sickle blood activities are important for a better management of the disease and reducing the medical cost. In this research, a mobile application controlled, impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. Calibration of the portable device is performed using a component of known impedance value. The preliminary test results are then compared to those obtained by a commercial benchtop impedance analyzer for further validation. With the developed portable flow cytometer, experiments are performed on two sickle cell samples and a healthy cell sample. The acquired results are subsequently analyzed with MATLAB scripts to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals are observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.

Extent

57 p.

Comments

Includes bibliography.|Thesis (M.S.)--Florida Atlantic University, 2018.|FAU Electronic Theses and Dissertations Collection|Includes bibliography.

Identifier

FA00013145

Format

pdf

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