Date of Award
2015
Document Type
Dissertation
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Beetle, Christopher
Abstract
Two photon microscopy is one of the fastest growing methods of in-vivo imaging of the brain. It has the capability of imaging structures on the scale of 1μm. At this scale the wavelength of the imaging field (usually near infra-red), is comparable to the size of the structures being imaged, which makes the use of ray optics invalid. A better understanding is needed to predict the result of introducing different media into the light path. We use Wolf's integral, which is capable of fulfilling these needs without the shortcomings of ray optics. We predict the effects of aberrating media introduced into the light path like glass cover-slips and then correct the aberration using the same method. We also create a method to predict aberrations when the interfaces of the media in the light-path are not aligned with the propagation direction of the wavefront.
Extent
69 p.
Recommended Citation
Estrada, Gerardo, "Improving In Vivo Two Photon Microscopy Without Adaptive Optics" (2015). Electronic Theses and Dissertations 2010 - 2019. 1241.
https://digitalcommons.fau.edu/etd_general_6/1241
Identifier
FA00004495
Comments
Includes bibliography.|Dissertation (Ph.D.)--Florida Atlantic University, 2015.|FAU Electronic Theses and Dissertations Collection|Includes bibliography.