Date of Award
2010
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Abstract
Microcystin-LR (MCLR) is hepatotoxic to animals and humans with disruption of liver structure causing cytoskeletal damage, necrosis and pooling of blood in the liver, leading to large increase in liver weight. It is also a strong liver tumor promoter and protein phosphatase inhibitor. Microcysin-LR binds protein phosphatases 1 and 2A, and influences regulation of cellular protein phosphorylation. In the present study, a colloidal gold based immunoassay test strip was developed for Microcystin-LR detection. The detection limit was found to be 1 ng/mL. 5 nm colloidal gold test strips exhibits more efficient for detection, compared with 20 nm colloidal gold test strips. The interaction between Microcystin-LR antibody (immunoglobulin G) and colloidal gold nanoparticles was investigated by various analytical methods, including Ultraviolet/Visible (UV/VIS), Fourier Transform Infrared (FTIR) and Fluorescence spectroscopy as well as transmission electron microscopy (TEM).
Extent
ix, 53 p. : ill. (some col.)
Recommended Citation
Xu, Jiesi., "Immunoassay test strip for Microcystin-LR detection" (2010). Electronic Theses and Dissertations 2010 - 2019. 107.
https://digitalcommons.fau.edu/etd_general_6/107
Identifier
FADT2683532
Comments
by Jiesi Xu.|Thesis (M.S.)--Florida Atlantic University, 2010.|Includes bibliography.|Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.|by Jiesi Xu.