Date of Award
2004
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Hartmann, James X.
Abstract
The purpose of this study was to determine the effect of bacterial lipopolysaccharide (LPS) on the expression of RP105 (CD180) in human B lymphocytes from normal, leukemic, and lupus patients. The percentage of cells that express RP105 on their surface increased following a 24 hour exposure to LPS. However, continued exposure for a total of four days resulted in a marked decrease in the expression of this receptor molecule. Human B cells were purified by a combination of density gradient and negative magnetic bead selection and maintained in culture with and without LPS. Enzyme linked immunoassay for the detection of anti-dsDNA antibodies following LPS treatment of isolated B cells was negative. The percentage of RP105 positive or negative B cells from lupus patients could not be accurately determined because too few B cells were available from these lymphopenic patients following negative selection.
Extent
28 p.
Recommended Citation
Nikolic, Veljko., "The role of bacterial lipopolysaccharide in the production of the lupus B cell phenotype" (2004). Electronic Theses and Dissertations 2000 - 2009. 789.
https://digitalcommons.fau.edu/etd_general_5/789
Identifier
FADT13177
Comments
Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 2004.|Charles E. Schmidt College of Science