Author Type

Graduate Student

Date of Award

2002

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Narayanan, Ramaswamy

Abstract

Recently Dr. Narayanan's laboratory, utilizing bioinformatics approaches, identified a novel gene which may play a role in colon cancer. This gene in view of its expression specificity was termed Colon Carcinoma Related Gene (CCRG). The CCRG belongs to a novel class of secreted molecules with a unique cysteine rich motif. The function of CCRG however, remains unknown. The basis of this project revolved around establishing the putative function (functional genomics) of CCRG. The rationale for the project was to test a hypothesis that CCRG may offer a growth advantage to cancer cells. The availability of diverse tumor-derived cell lines, which were CCRG negative offered a possibility to study the consequence of enforced expression of CCRG. A breast carcinoma cell line was transfected with an exogenous CCRG expression vector and the stable clones were characterized. The stable transfectants of CCRG showed enhanced growth and a partial abrogation of serum growth factor(s) requirement. These results provide a framework for future experiments to further elucidate the function of CCRG.

Extent

64 p.

Comments

Thesis (M.S.)--Florida Atlantic University, 2002.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 2002.

Identifier

FADT12965

Format

pdf

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