Date of Award
2000
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Hartmann, James X.
Abstract
This study established a lateral flow competitive immunoassay with a colloidal gold-monoclonal antibody probe for the qualitative detection of sailfish serum albumin. The test involves mixing a tissue homogenate with a colloidal gold-monoclonal antibody suspension and applying the mixture to a strip of plastic-backed nitrocellulose membrane. The presence of albumin in a target sample competed with adsorbed antigen and prevented the appearance of a pink color on the nitrocellulose membrane. A non-target sample yielded a pink color when gold-labeled monoclonal antibodies bound to sailfish albumin previously absorbed to the nitrocellulose. Three gold particle sizes for antibody conjugation were evaluated and of these, 41 nm was optimal. The optimal pH for conjugation of anti-sailfish antibody to colloidal gold was 7.0. The assay requires only five minutes to perform and utilizes two solutions.
Extent
64 p.
Recommended Citation
Duan, Fei., "Colloidal gold labeling of monoclonal antibodies for billfish species identification" (2000). Electronic Theses and Dissertations 2000 - 2009. 18.
https://digitalcommons.fau.edu/etd_general_5/18
Identifier
FADT15789
Comments
Thesis (M.S.)--Florida Atlantic University, 2000.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 2000.