Date of Award
2009
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Abstract
The zinc finger associated domain (ZAD) containing family of transcription factors is not well described in the literature, in part because it is very difficult to study by mutagenesis. We used in vitro-binding techniques to identify characteristics of the ZAD family, by constructing glutathione Stransferase (GST)-ZAD domain chimeric proteins for use in protein binding assays, and GST-Zinc finger array domain chimera for binding site selections. Protein binding assays indicated a possible shared cofactor, as seen in the analogous KRAB system in mammals. DNA binding assays have provided a consensus binding sequence for five of the ZAD proteins, consistent with previously reported work on ZAD and unpublished work on mammalian transcription factors. Research is ongoing with an additional ~50 ZAD proteins to more fully map the binding characters of ZAD proteins.
Extent
vii, 49 p. : ill. (some col.).
Recommended Citation
Krystel, Joseph., "Elucidation of the features of the zinc finger associated domain (ZAD) family of transportation factors in Drosophila melanogaster" (2009). Electronic Theses and Dissertations 2000 - 2009. 1665.
https://digitalcommons.fau.edu/etd_general_5/1665
Identifier
FADT186768
Comments
by Joseph Krystel.|Thesis (M.S.)--Florida Atlantic University, 2009.|Includes bibliography.|Electronic reproduction. Boca Raton, Fla., 2009. Mode of access: World Wide Web.|by Joseph Krystel.