Date of Award
2007
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Zhang, Xing-Hai
Abstract
The thesis consists of two chapters, and within each chapter is a different set of methods and techniques that will be useful to me in future research endeavors. The first is the transformation of tobacco chloroplast and the analysis of a resultant chloroplast mutant, and the second is the establishment of a regeneration system for the aquatic plant Typha domingensis, commonly known as cattail. The unifying theme is plant transformation. The establishment of a regeneration system for a potentially beneficial plant is useful for future transformations and the actual transformation and analysis of mutants is useful for the characterization of transformants. The chloroplast transformation was unsuccessful and analysis of the mutation demonstrated it as a null mutation under normal growth conditions. Cattail seeds were induced to form calli, then induced to regenerate into normal plants.
Extent
44 p.
Recommended Citation
Tapia, Claudius E., "Molecular Characterization of a Nicotiana tabacum Chloroplast Mutant and in vitro Regeneration of a Cattail, Typha domingensis, from Embryonic Callus" (2007). Electronic Theses and Dissertations 2000 - 2009. 1308.
https://digitalcommons.fau.edu/etd_general_5/1308
Identifier
FA00000839
Comments
Includes bibliography.|Thesis (M.S.)--Florida Atlantic University, 2007.|FAU Electronic Theses and Dissertations Collection|Charles E. Schmidt College of Science|Includes bibliography.