Date of Award
2007
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Caputi, Massimo
Abstract
The RRE is a sequence of the HIV genome which is required for the export of the unspliced mRNA from the nucleus to the cytoplasm. Previous studies show that RRE on the HIV mRNA binds directly to Rev which then interacts with Ran and CRM 1 to form an export complex. Our results indicate that Ran can interact with the RRE in the absence of Rev and CRM 1 but in the presence of other factor(s) present in the nuclear extract. Ran-GEF or RCC 1 seems to be a potential mediating factor. Our results suggest that Ran binds directly to RCC 1 and that the binding is disrupted by addition of excess nucleotides and magnesimn. Our suggestion is that Ran and RCC 1 are members of an alternate export pathway present in the HIV. Our observation that the binding is nonspecific makes us speculate that this export pathway may be present in other cell types as well.
Extent
85 p.
Recommended Citation
Dhir, Neetika, "Characterization of Cellular Proteins Binding HIV's Rev Responsive Element (RRE)" (2007). Electronic Theses and Dissertations 2000 - 2009. 1228.
https://digitalcommons.fau.edu/etd_general_5/1228
Identifier
FA00000742
Comments
Includes bibliography.|Thesis (M.S.)--Florida Atlantic University, 2007.|Charles E. Schmidt College of Medicine|FAU Electronic Theses and Dissertations Collection|Includes bibliography.