Date of Award
2006
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Zhang, Xing-Hai
Abstract
One methionine sulfoxide reductase A (TMSRA) and two methionine sulfoxide reductase Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2 could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while TMSRB 1 showed little activity with TRX but much more activity with DTT, which was similar to the mitochondrial MSRB2 from mammals. Ferredoxin (FD) is not the reducing system for Msrs, but might reflect the redox status in the cell and control the activity of Msrs indirectly.
Extent
55 p.
Recommended Citation
Ding, Di, "Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant" (2006). Electronic Theses and Dissertations 2000 - 2009. 1012.
https://digitalcommons.fau.edu/etd_general_5/1012
Identifier
FA00000746
Comments
Includes bibliography.|Thesis (M.S.)--Florida Atlantic University, 2006.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Includes bibliography.