Author Type

Graduate Student

Date of Award

1994

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Hartmann, James X.

Abstract

A monoclonal antibody (MAb) produced against sailfish (Istiophorus albicans) serum albumin (SFA)/(Rossi et al. 1992) was used in conjunction with rabbit polyclonal antibodies (PAbs) to formulate a sandwich-style enzyme immunoassay (sEIA). The sEIA specifically identifies sailfish tissues utilizing samples such as heart, kidney, white muscle, red muscle, and blood serum. The assay is sensitive to 20ng of SFA. Of the tissues tested, kidney yields the strongest signal. The entire assay is carried out in a 3cc syringe and, excluding sample preparation, can be executed in 30 minutes. No specialized equipment or training is required. MAbs were also produced against serum albumin purified from white marlin (Tetrapturus albidus) and Atlantic blue marlin (Makaira nigricans). These Mabs were examined for their utility in identification assays for these species.

Extent

92 p.

Comments

Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 1994.|Charles E. Schmidt College of Science

Identifier

FADT15052

Format

pdf

Download

Share

COinS