Date of Award
1992
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Haky, Jerome E.
Abstract
The performance of several alkyl-bonded alumina-based stationary phases was evaluated by comparing the separation of synthetic octapeptide and polypeptide mixtures and tryptic digests of larger proteins. These phases were of differing pore diameter, alkyl chain length modification and particle shape and size. The separations were compared to standard silica phases. The narrow pore octadecyl bonded alumina phase outperformed the other alumina and silica phases in terms of separation efficiency and mobile phase resistance. Superior performance is attributed to the enhanced solute mass transfer properties and the unique morphology of the microplatelet alumina particles. The mechanism of separation gradually changes with increasing size of the peptide.
Extent
157 p.
Recommended Citation
Ramdial, Nirmala Debra-Ann, "Comparison of alkyl-bonded alumina-based stationary phases for peptide separation by high performance liquid chromatography" (1992). Electronic Theses and Dissertations 1990 - 1999. 273.
https://digitalcommons.fau.edu/etd_general_4/273
Identifier
FADT14831
Comments
Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 1992.|Charles E. Schmidt College of Science