Date of Award
1991
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Haky, Jerome E.
Abstract
Alumina-based stationary phases are evaluated for the separations of proteins and peptides by reversed phase high performance liquid chromatography. Separations are compared to those obtained on a widely-used octadecylsilane (ODS) phase. The separations of peptides on alumina-based stationary phases are found to be superior while separations of proteins are inferior as compared to those found on ODS phase. The superior performance of peptide separations on alumina-based columns is attributed to lower pore size and uniquely-shaped particles of the alumina. The retentions of peptides and proteins on both alumina and silica-based stationary phases are shown to be governed by hydrophobic interaction mechanisms.
Extent
117 p.
Recommended Citation
Raghani, Anil Ratilal, "Evaluation of alumina-based stationary phases for the separations of proteins and peptides by high performance liquid chromatography" (1991). Electronic Theses and Dissertations 1990 - 1999. 174.
https://digitalcommons.fau.edu/etd_general_4/174
Identifier
FADT14719
Comments
Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 1991.|Charles E. Schmidt College of Science