Author Type

Graduate Student

Date of Award

1998

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Hartmann, James X.

Abstract

Polystyrene bead and paddle formats were tested for their efficacy in an immunoassay for identification of sailfish. Different parameters were evaluated for their ability to prevent non-specific reactions in an indirect immunoassay utilizing underivatized polystyrene beads. Blocking agents which worked well in a polystyrene plate-based assay proved unsuccessful with the polystyrene bead-based assay. A major drawback to the use of a bead-based assay was the necessity of using multiple beads for the identification of a single sample. It was shown that the Nunc-Immuno$\rm\sp{TM}$ Stick format could be adapted for use in a sailfish identification assay that yielded a visible color reaction. The main benefit of using this format is the ability to perform the entire assay with positive and negative controls in a single tube. Color development in the Nunc-Immuno$\rm\sp{TM}$ stick format was eluted and the optical density difference between target (0.77 average) and non-target species (0.12 average) was highly significant.

Extent

52 p.

Comments

Thesis (M.S.)--Florida Atlantic University, 1998.|Thesis (M.S.)--Florida Atlantic University, 1998.

Identifier

FADT15592

Format

pdf

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