Date of Award
1998
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Kerr, Russell G.
Abstract
The fatty acid synthetase (FAS) from the marine invertebrate Bugula neritina was isolated using cold ethanol precipitation followed by the sequence of G-100 and G-200 size exclusion columns. Native gel analysis indicates the isolation of the FAS and the elution volume from the G-100 column suggests the FAS to be ~282kd. One band with a molecular weight of 66 kDa appeared on the SDS gel of the G-200 sample (F17-30) that eluted at 52.5 mL. The G-200 sample that eluted at 19.2 mL (F1-6) displayed two predominant bands on the SDS gel corresponding to molecular weights 66 kDa and 97 kDa. Both F1-6 and F17-30 showed FAS activity displaying de novo production of myristic and palmitic acids. From the sequence of purification starting from the cell-free extract (CFE) to the F17-30 sample, a 240 fold increase in specific activity was observed. The Type II FAS experiments showed no substantial evidence of activity, namely of the beta-Hydroxybutyryl acyl-dehydrase and the enoyl reductase enzymes.
Extent
83 p.
Recommended Citation
Koh, Francis H., "Isolation of the fatty acid synthetase from the marine invertebrate Bugula neritina" (1998). Electronic Theses and Dissertations 1990 - 1999. 1327.
https://digitalcommons.fau.edu/etd_general_4/1327
Identifier
FADT15546
Comments
Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 1998.|FAU Electronic Theses and Dissertations Collection|Charles E. Schmidt College of Science