Author Type

Graduate Student

Date of Award

1998

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Hartmann, James X.

Abstract

This study describes an affinity method which purifies albumin from fish serum. Hybridoma clones producing antibodies specific to fish albumin have been screened and propagated and their antibodies coupled to a cross-linked agarose gel matrix which has been activated with N-hydroxysuccinimide. The resulting gel with its ligand attached was then used to purify albumin from fish serum. The procedure was optimized for coupling, sample application and elution. The coupling optimization using varying pH values resulted in over 90% coupling efficiencies. The sample application optimization showed yields of 2 mg of protein per ml of serum at 1:5 dilution of sample. Elution conditions were evaluated using varying pH and chaotropic agent concentrations. The optimal elution conditions (pH 4.0) resulted in a yield of 0.2 mg/ml of gel with several impurities. Elution at pH 3.0 resulted in a very pure product with low yield (.02 mg/ml of gel) and a loss of activity.

Extent

51 p.

Comments

Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 1998.|FAU Electronic Theses and Dissertations Collection|Charles E. Schmidt College of Science

Identifier

FADT15568

Format

pdf

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