Author Type

Graduate Student

Date of Award

1998

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Hartmann, James X.

Abstract

A fusion was conducted using spleen cells from a hyperimmunized Balb/C mouse and a murine myeloma cell line, in an attempt to create monoclonal antibodies specific to parvalbumin from Centropomus undecimalis. The fusion protocol utilized a 24 well culture plate protocol. Initial results indicated the successful generation of antibody producing hybridomas specific to the desired parvalbumin; however, attempts to isolate and clone these cells were unsuccessful. The study results clearly demonstrate some potential drawbacks to using the 24-well fusion protocol versus a 96-well protocol. The 24-well protocol seems to favor rapidly growing non-secreting cells due to the lower dilutions involved. This study was successful in isolating murine polyclonal antiserum reactive in isolating murine polyclonal antiserum reactive with piscine parvalbumin. Further, initial cultures of hybridomas were able to distinguish C. undecimalis from several other species of fishes thus confirming the suitability of parvalbumin as a biomaker protein.

Extent

51 p.

Comments

Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 1998.|Charles E. Schmidt College of Science

Identifier

FADT15552

Format

pdf

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