Date of Award
1998
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Kerr, Russell G.
Abstract
9,11-Secosteroids exhibit novel biological activity and interesting pharmacological properties. A biosynthetic study of 9,11-secogorgosterol and other novel 9,11-secosteroids using an enzyme extract from the gorgonian Pseudopterogorgia americana demonstrated that this system is an efficient catalyst for a wide range 9,11-secosteroid production. Two methods to recover the enzyme from 9,11-secosteroid synthesis have been developed: (1) membrane-enclosed enzymatic catalysis, which combines certain of the advantages of soluble enzymes and immobilized enzymes, shows high activity, simple recovery and at least 5 cycles or 23 days; (2) covalently bonded enzyme-agarose gel, attached by cyanogen bromide via the amino groups of enzymes, show 80% activity of free enzymes and at least 7 cycles or 46 days. Based on stability and kinetic studies of these immobilized enzymes in 9,11-secosteroid synthesis, a continuous flow column reactor for scale-up of 9,11-secosteroid production has been developed with great efficiency, which represents the first enzymatic production of a bioactive compound from a marine invertebrate on a large scale.
Extent
77 p.
Recommended Citation
Chen, Ying, "Development of methods for the immobilization of enzymes used in 9,11-secosteroid biosynthesis" (1998). Electronic Theses and Dissertations 1990 - 1999. 1286.
https://digitalcommons.fau.edu/etd_general_4/1286
Identifier
FADT15521
Comments
Thesis (M.S.)--Florida Atlantic University, 1998.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 1998.