Author Type

Graduate Student

Date of Award

1998

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Hartmann, James X.

Thesis/Dissertation Co-Chair

Mari, Frank

Abstract

Parvalbumins are acidic calcium-binding proteins found in large quantities in the white muscle fibers of cold-blooded vertebrates. Fish display two to seven parvalbumin isotypes that are species specific and thus constitute a valuable tool in the study of phylogenetic relationships, and as a specific biomarker for fish identification. Parvalbumins were isolated from a selected sample of marine teleosts and elasmobranchs. Purified isotypes were characterized via HPLC (gel filtration, and ion exchange), and electrophoresis (IEF, and SDS-PAGE). An indirect immunoassay was developed for the parvalbumin isotypes using monoclonal antibodies directed against the highly conserved calcium-binding site. Parvalbumins from selected fish species are compared and contrasted by standard biochemical and immunological methods.

Extent

223 p.

Comments

FAU Electronic Theses and Dissertations Collection|Thesis (M.Sc.)--Florida Atlantic University, 1998.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection

Identifier

FADT15536

Format

pdf

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