Author Type

Graduate Student

Date of Award

1989

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Abstract

Influenza virus type A neuraminidase (NA) was detected in the supernatant of virus infected monkey kidney (Vero) and canine kidney (MDCK) cell cultures utilizing isoelectric focusing (IEF) techniques and a fluorogenic substrate, 4-methylumbelliferol-alpha-N-neuraminic acid (4-MUN). Lipids were extracted from the virus infected cells by phase separation in the organic solvent Lipo-clean. The aqueous phase was sonicated in the presence of the detergent NP-40 in order to solubilize the proteins. Concentration of proteins by ultrafiltration and separation by IEF on the PhastSystem (Pharmacia) followed. Proteins from the IEF gel were blotted to nitrocellulose which had been soaked in the 4-MUN substrate solution. Fluorescent bands specific for influenza type A viral NA were detected with a long range UV light. This technique may have applications for use with clinical respiratory specimens since it has the capability to distinguish between bacterial and viral neuraminidases, including type A and B influenza and parainfluenza viruses.

Extent

41 p.

Comments

Adviser: James X. Hartmann.|Thesis (M.S.)--Florida Atlantic University, 1989.|Adviser: James X. Hartmann.

Identifier

FADT14571

Format

pdf

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