Author Type

Graduate Student

Date of Award

1986

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Abstract

Successful cryogenic preservation of semen from a psittacine species, Melopsittacus wzdulatus, was first achieved in this study. A total of 64 combinations of the following variables were tested, using percent motility as the criterion of successful freezing: two diluents (Beltsville Poultry Semen Extender (BPSE) and acetate); two dilution ratios; four different holding times (time that semen plus diluent equilibrate); and four different equilibration times (time that semen and diluent equilibrate with cryoprotectant), with a significant interaction occuring between these variables. Two different levels of dimethylsulfoxide (DMSO) were also tried. Five combinations of these variables yielded mean motilities >70 % upon thawing, all using dilution ratios of 1:2; four had acetate as the diluent and the fifth had BPSE. Successful artificial insemination (AI) using the frozen - thawed semen may have been achieved in one instance using the acetate diluent, 10% DMSO (v/ v), a 1:2 dilution ratio, a holding time of 60 min and an equilibration time of 45 min: 1 of 2 eggs produced was fertile.

Extent

48 p.

Comments

Thesis (M.S.)--Florida Atlantic University, 1986.|Thesis (M.S.)--Florida Atlantic University, 1986.

Identifier

FADT14323

Format

pdf

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