Date of Award
1985
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Stein, Abraham
Abstract
The objective of this study was to elucidate the interaction of the carcinogen MNNG with nuclear DNA of Rat Hepatoma cells. The effect of a range of MNNG concentrations on RH cell DNA was studied by an analysis of the DNA fragments obtained in linear alkaline sucrose gradients. A macromolecular analysis of the sedimentation profile for 0.06 mM MNNG (Experiment I), and 0.1 mM MNNG (Experiment III), suggested that the system was paucidisperse and contained at least three components. The major component Max 1 has a molecular weight comparable to that known for the mammalian replicon. Max 2 has a molecular weight twice that of Max 1 and Max 3 has a molecular weight half that of Max 1. The size of the replicon is comparable to that obtained by others. Inferences were drawn regarding the structure of chromatin and the role of the distribution of sites hypersensitive to methylation with respect to the oncogenes.
Extent
213 p.
Recommended Citation
JHABVALA, PERSEUS, "EFFECT OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE (MNNG) ON DNA OF CULTURED RAT HEPATOMA CELLS" (1985). Electronic Theses and Dissertations 1980 - 1989. 364.
https://digitalcommons.fau.edu/etd_general_3/364
Identifier
FADT14260
Comments
Thesis (M.S.)--Florida Atlantic University, 1985.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 1985.