Author Type

Graduate Student

Date of Award

1981

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Hartmann, James X.

Abstract

Interferon (IFN) production has been studied in fish cell cultures using channel catfish virus as an inducer. Two established cell lines, brown bullhead (BB) and walking catfish gill cells (GlB) were compared for their IFN releasing capacity with two virus strains, ATCC5 (wild) and v60 (vaccine) type. IFN was assayed by the plaque count method in BB cells using ATCC5 as challenge virus. The IFN, as an unpurified culture fluid, was found to be: stable over a wide range of pH 2-10; heat stable at 37 C for one hour; nondializable; resistant to Rnase and Dnase action; susceptible to trypsin, pepsin, and protease degradation; nontoxic to cells and unable to inactivate virus directly. Thus, the product has fulfilled most of the basic criteria for it to be classified as interferon. This study indicated that all four cell/virus interferon products caused a plaque reduction of approximately 90-97% in a homologous system.

Extent

102 p.

Comments

Thesis (M.S.)--Florida Atlantic University, 1981.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 1981.

Identifier

FADT14063

Format

pdf

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