Author Type

Graduate Student

Date of Award

1979

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Schultz, Franklin A.

Abstract

Analysis of creatine phosphokinase (CPK) isoenzymes is the best clinical test for diagnosis of heart disease. Two new methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies. These new methods and the conventionally used technique of electrophoresis are comparatively evaluated using assayed controls and cardiac patient serum samples. Results indicate that thiol activation and antibody inhibition are preferred methods for CPK cardiac isoenzyme analysis because they have lower levels of detection, fewer false negative results, and are considerably more efficient than electrophoresis. The new techniques also are adapted to automated spectrophotometric instrumentation, which further contributes to their accuracy and procedural efficiency. Thus, thiol activation and antibody inhibition methods should provide more sensitive and reliable CPK isoenzyme analysis for critical supportive evidence in heart disease diagnosis and treatment

Extent

101 p.

Comments

Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 1979.|FAU Electronic Theses and Dissertations Collection|Charles E. Schmidt College of Science

Identifier

FADT13982

Format

pdf

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