Author Type

Graduate Student

Date of Award

1977

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Abstract

Plant cells and protoplasts were frozen and maintained at -196 C for five days. After thawing at 37 C their viability was assessed by trypan blue dye exclusion. Protoplasts were isolated by enzymatic digestion of the cell walls of two day cultures of Bromus inermus (Leyss), Daucus carota (L.), and Glycine max (L.) Merr. The survival of brome grass protoplasts was 59.45% ± 3.59, that of carrot 52.09% ± 18.99 in 1.4 M and 0.7 M glycerol respectively. With cells the survival was 35.64% ± 2.07 for brome grass and 39.45% ± 2.47 for carrot. Protoplasts frozen in a three step protocol (+4, -20, -58 C) exhibited 10% greater survival than those frozen directly at -58 C. The isolation of protoplasts in medium containing 0. 7 M glycerol was beneficial. A direct relationship between cell wall removal and an increase in protoplast survival was evident. Protoplasts which had been frozen (-196 C) for five days can be cultured as evidenced by cell wall regeneration, cell division, and cyclosis.

Extent

58 p.

Comments

Thesis (M.S.)--Florida Atlantic University, 1977.|Thesis (M.S.)--Florida Atlantic University, 1977.

Identifier

FADT13871

Format

pdf

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