Author Type

Graduate Student

Date of Award

1975

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Abstract

The effectiveness of polyethylene glycol as a fusogenic agent tor avian erythrocytes was evaluated. Parameters affecting the efficiency of fusion included number of pre-fusion washes, age of cells, length of exposure to enzyme solutions at varied pH values, composition of wash solutions and molecular weights of polyethylene glycol. Fusion was measured as the percentage of visible polykaryons. Maximal fusion obtained using polyethylene glycol was approximately 60-70%. Other methods of cell fusion (lysolecithin, Sendai virus, high calcium concentration at high pH) were compared to polyethylene glycol. All other means yielded lesser amounts of fused products. A photographic comparison of the various methods is presented, and possible mechanisms of fusion are discussed.

Extent

85 p.

Comments

Thesis (M.S.)--Florida Atlantic University, 1975.|Thesis (M.S.)--Florida Atlantic University, 1975.

Identifier

FADT13703

Format

pdf

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