Date of Award
1973
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Hoffmann, H. A.
Abstract
Two methods, a membrane filter-fluorescent antibody technique for detection of Staphylococcus aureus, and isotopic determination of relative heterotrophic activity, were compared with a standard technique based on the recovery of coliform organisms for evaluation as indices of marine pollution. Water samples were collected from coastal areas of Pompano Beach and Boca Raton, Florida. Coliform and staphylococci survival in seawater was studied under laboratory conditions. The effect of exposure to seawater on substrate uptake by coliforms and other sewage microorganisms was studied using isotopic methods. The results of these studies support the feasibility of using S· aureus as a long-range indicator of marine pollution. Under natural conditions, S· aureus appears more resistant to seawater than coliform organisms, although the reverse was found under laboratory conditions. Seawater inhibited uptake of glucose and lactose by Escherichia coli and other sewage organisms. Relative heterotrophic potentials in terms of glucose and lactose uptake velocities were not significantly affected in coastal waters by the influx of bacteria and nutrients in sewage outfall effluent.
Extent
51 p.
Recommended Citation
BUCHANAN, IAN ELLIS, "EVALUATION OF MICROBIOLOGICAL METHODS FOR USE IN MARINE WATERS: ENUMERATION OF STAPHYLOCOCCI BY A FLUORESCENT ANTIBODY TECHNIQUE, AND ISOTOPIC DETERMINATION OF BACTERIAL METABOLIC POTENTIAL" (1973). Electronic Theses and Dissertations 1970 - 1979. 187.
https://digitalcommons.fau.edu/etd_general_2/187
Identifier
FADT13557
Comments
Thesis (M.S.)--Florida Atlantic University, 1973.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 1973.