Date of Award
1973
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Saurino, Vincent R.
Abstract
Leukocytes, harvested from EIA infected horse plasma, were disrupted and partially purified by differential centrifugation. This material was assayed for its capacity to inhibit migration of leukocytes from infected horses. After 6 X 10^6 r of gamma radiation the material was reassayed and 85% of the initial activity remained, indicating that little antigenic destruction had occurred. This material was coupled to rabbit gamma globulin and used for horse inoculation. The above material, uncoupled, and a partially purified irradiated normal horse protein material were used as controls. Responses to immunizations and challenges were monitored. Uncoupled irradiated material produced the disease. Coupled irradiated material did not produce the disease and the immunized horse was resistant to challenge with 1 ml of a 10^-3 dilution of infectious serum for 30 days and, then, to 1 ml of undiluted infectious serum for 30 more days .
Extent
86 p.
Recommended Citation
BELTRANENA, HUMBERTO PEDRO PABLO, "EVALUATION OF A GAMMA-IRRADIATED, GAMMA GLOBULIN COUPLED, PARTIALLY PURIFIED MATERIAL AS AN IMMUNIZING AGENT FOR EQUINE INFECTIOUS ANEMIA (EIA)" (1973). Electronic Theses and Dissertations 1970 - 1979. 185.
https://digitalcommons.fau.edu/etd_general_2/185
Identifier
FADT13566
Comments
Thesis (M.S.)--Florida Atlantic University, 1973.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 1973.