Author Type

Graduate Student

Date of Award

1972

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Abstract

Cellulolytic enzymes were induced in the marine fungal isolates, Culcitalna achraspora, Halosphaeria mediosetigera, and Zalerion xylestrix by native cotton and related substrates. Marine environmental conditions were maintained in an artificial seawater medium. Induced c1 and ex enzymes and cellobiase were determined colorimetrically. Induction also occurred on cellobiose, but not on glucose and lactose. Permenter studies with glucose and with toilet tissue carbon sources gave close correlations between mycelial dry weight, enzyme component activity, and cell nitrogen. In cotton substrate media, Tween 80 increased C1 activity by 148%, and Cx activity by 160%, on the average. Millipore ultrafiltration was the best technique for concentrating cellulase for Sephadex G-75 column chromatography, while conventional techniques were unsuccessful. Fractionation diagrams for all cultures were similar with 2 distinct peaks. The low molecular weight fraction contained mainly Cx enzyme activity, while the high molecular weight fraction contained C1 and Cx enzymes and cellobiase. These fungi possess a cellulase similar, but not identical, to that found in terrestrial species.

Extent

73 p.

Comments

Thesis (M.S.)--Florida Atlantic University, 1972.|Thesis (M.S.)--Florida Atlantic University, 1972.

Identifier

FADT13537

Format

pdf

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