Date of Award
1972
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Abstract
Water, Hoagland solution, glucose solutions, kinetin and benzimidazole were tested for their ability to delay senescence of detached Eichhornia crassipes leaves. Water and Hoagland solution failed to sustain chlorophyll past 9 days. Glucose solutions supported contaminating microbial growth and were judged unsatisfactory. Kinetin and benzimidazole solutions maintained a "normal appearance" in detached leaves beyond three weeks culture. Determination of the conversion of protein to amino acids and amines as well as carbohydrate levels in detached leaf culture indicated the optimal culture media were 100 ppm benzimidazole and benzimidazole-kinetin mixture, 50 ppm each. The effective length of culture was three weeks. The utility of this leaf culture system as an alternative to intact host inoculation with pathogenic microorganisms is demonstrated by the isolation and subculture of a pathogenic Alternaria in E. crassipes leaves supported by 50 ppm benzimidazole solution.
Extent
63 p.
Recommended Citation
CAPPLEMAN, LAWRENCE EUGENE JR, "DETACHED LEAF CULTURE OF EICHHORNIA CRASSIPES AND APPLICATION TO THE CULTURE OF ITS PATHOGENS" (1972). Electronic Theses and Dissertations 1970 - 1979. 105.
https://digitalcommons.fau.edu/etd_general_2/105
Identifier
FADT13531
Comments
Thesis (M.S.)--Florida Atlantic University, 1972.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 1972.