Date of Award
1972
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Abstract
The effects of added salt on the deacylation rate constant of trans-cinnamoylchymotrypsin were examined. Ionic strength effects on the equilibrium constant of an ionizable, enzymic group of pKa 7.25 were also studied. Through the development of methods for the precise measurement of pH and observed rate constants, very small changes in the experimental parameters could be detected. It was found that neither the deacylation rate constant nor the kinetically determined pKa exhibited a significant salt effect over the ionic strength range of 0.04 to 0.12 M. Based on the known three-dimensional structure of the active site of chymotrypsin, four plausible mechanisms were considered in light of the experimental absence of salt effects. The presence of calcium ion at low concentrations significantly perturbed the values of the deacylation rate constant and the protonic equilibrium constant.
Extent
83 p.
Recommended Citation
COLEMAN, DANIEL R., "CHARGE DISTRIBUTION IN CHYMOTRYPSIN CATALYSIS" (1972). Electronic Theses and Dissertations 1970 - 1979. 100.
https://digitalcommons.fau.edu/etd_general_2/100
Identifier
FADT13496
Comments
Thesis (M.S.)--Florida Atlantic University, 1972.|Charles E. Schmidt College of Science|FAU Electronic Theses and Dissertations Collection|Thesis (M.S.)--Florida Atlantic University, 1972.