Author Type

Graduate Student

Date of Award

2022

Document Type

Thesis

Publication Status

Version of Record

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Waseem Asghar

Abstract

Exosomes have gained recognition in cancer diagnostics and therapeutics. Most exosome isolation methods are time-consuming, costly and require bulky equipment, rendering them unsuitable for point-of-care (POC) settings. Microfluidics can be the key to solving these challenges. Here, we employ the development of a double filtration microfluidic device that can rapidly isolate exosomes in POC settings. The device can efficiently isolate exosomes from just 100 uL of plasma within 50 minutes. The device was compared against Polyethylene glycol (PEG) based precipitation, and findings show that both methods yield comparable exosome sizes and purity, but the device can detect exosomal miRNA earlier than PEG. Finally, a comparative analysis of membrane filters with exosomes collected from pore sizes 15 nm and 30 nm showed a similarity in exosome size and miRNA expressions, with significantly increased sample purity. These findings suggest that this device has potential in POC settings.

Extent

70 p.

Comments

Includes bibliography.|Thesis (MS)--Florida Atlantic University, 2022.|FAU Electronic Theses and Dissertations Collection|Includes bibliography.

Identifier

FA00014071

Format

pdf

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