Author Type

Undergraduate Student

Date of Award

Fall 12-1-2024

Document Type

Thesis

Publication Status

Version of Record

Submission Date

August 2026

College Granting Degree

Harriet L. Wilkes Honors College

Thesis/Dissertation Advisor [Chair]

Kelsie Bernot

Thesis/Dissertation Co-Chair

Tracy Mincer

Abstract

Recent studies have revealed Anaplasma phagocytophilum as a new pathogen that has started to affect gopher tortoise populations throughout Florida. This study investigated how the pathogen, A. phagocytophilum, has potentially begun to spread amongst the gopher tortoises in the Abacoa Greenway and the Florida Atlantic University Preserve (FAUP). To investigate these tortoises, blood samples were taken from multiple specimens from Range VI of the Greenway and FAUP. DNA isolated from these samples were then analyzed for the presence of A.phagocytophilum utilizing PCR and gel electrophoresis, followed by Sanger sequencing. This research demonstrated that A.phagocytophilum has not made its way to the Greenway or FAUP. These findings will hopefully contribute vital information to help maintain gopher tortoise populations throughout southeast Florida.

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