Analysis of Mutant Manganese-Superoxide Dismutase on the Lifespan of Saccharomyces cerevisiae""

Author

• Sarah Salem

Date of Award

2011

Document Type

Dissertation/Thesis

Submission Date

October 2026

Thesis/Dissertation Advisor [Chair]

Kirchman, Paul A.

Thesis/Dissertation Co-Chair

Wetterer, James

Abstract

Previously isolated mutants of the superoxide dismutase 2 gene (SOD2), selected for increased activity in E. coli, were transformed into yeast cells in order to observe their effects on aging. Polymerase chain reaction and DNA sequencing were used to confirm that the yeast incorporated the mutant form of the gene. The strains with mutant forms of the SOD2 gene were observed to have decreased growth rates compared to the unmutated strain. Lifespan analyses were then conducted in order to see if yeast with mutant versions of SOD2 had lifespans that differed significantly from those with the unmutated forms of the gene. The mutant forms of SOD2 had no significant effect on the lifespan of yeast.

Comments

Includes bibliography.

Recommended Citation

Salem, Sarah, "Analysis of Mutant Manganese-Superoxide Dismutase on the Lifespan of Saccharomyces cerevisiae""" (2011). Harriet L Wilkes Honors College Theses 2010 - 2019. 83.
https://digitalcommons.fau.edu/etd_honors_2/83

Extent

26 p.

Identifier

FA00003604