Date of Award
2012
Document Type
Dissertation/Thesis
Submission Date
October 2026
Abstract
In my thesis work, I attempted to construct a plasmid that would allow stable integration of genes into the Saccharomyces cerevisiae yeast genome under the control of the repressible TetO promoter. The yeast ACO1 gene was cloned under the control of the TetO operator and the tTA transactivator. This construct was inserted into yeast cells in order to observe the effects of aconitase overexpression on aging. Unfortunately, the transformed cells appeared incapable of aconitase expression as determined by glutamic acid auxptrophy, a phenotype of aconitase mutants. We have sequenced the pIT1ACO1 plasmid and have found many abnormalities in the promoter region. If the plasmid can be made to function as intended, the resulting yeast strain can be used in the future to determine if aconitase plays an important role in cellular aging.
Recommended Citation
Nunes, Steve., "Creation of an aconitase overexpression strain of Saccharomyces cerevisiae for lifespan analysis" (2012). Harriet L Wilkes Honors College Theses 2010 - 2019. 116.
https://digitalcommons.fau.edu/etd_honors_2/116
Extent
v, 20 p. : ill. (some col.)
Identifier
819413214
Comments
by Steve Nunes.