Date of Award
1996
Document Type
Thesis
Publication Status
Version of Record
Submission Date
October 2026
Thesis/Dissertation Advisor [Chair]
Hartmann, James X.
Abstract
Spleenocytes from Balb/c mouse immunized with purified blue marlin albumin were fused with X 63 myeloma cells in a polyethylene glycol aided fusion. The resulting fusomas were screened for anti-blue marlin activity and a potential blue marlin specific hybridoma clone designated 605D was isolated and expanded in cell culture. While testing the reactivity of monoclonal antibody secreted by clone 605D against pooled serum samples of blue marlin, white marlin and sailfish in indirect ELISA, 605D could differentiate blue marlin from white marlin and sailfish clearly. The crossreactivity to white marlin was minimised by diluting sera samples and antibody solutions. However, when individual billfish serum samples and heart tissue homogenates were tested 605D could not differentiate between the two marlin species, though crossreactivity to SF was minimal.
Extent
57 p.
Recommended Citation
Paniker, Lakshmi., "Monoclonal antibodies and blue marlin (Makaira nigricans) identification" (1996). Electronic Theses and Dissertations 1990 - 1999. 966.
https://digitalcommons.fau.edu/etd_general_4/966
Identifier
FADT15347
Comments
Charles E. Schmidt College of Science|Thesis (M.S.)--Florida Atlantic University, 1996.|Charles E. Schmidt College of Science